CGSC Strain#: 3523
Strain Designation: AB3523     
Source of Strain: J.A. Wechsler
Other Designations: JW 41
Sex: F'
Episome/Plasmid: F2016-2
Plasmid Markers/Mutations: ilvD75,
ilvE ,
ilvA Chromosomal Markers: lacY1 or lacZ4,
galK2(Oc)
,
trpE3(Oc)
,
hisG4(Oc)
,
ilvD75,
ΔargH1,
thiE1Strain Comments: - This strain may carry an unmapped weak amber suppressor.
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- ilvD75-- suppressed by sup-13
- galK2(Oc) was formerly called gal2
- thiE1 was formerly called thi-1
- Oc = ochre(UAA) mutation
References: - Wechsler, J.A. 1969. Antipolarity in the ilv operon of Escherichia coli K-12. J.Bacteriol. 98:1179-1194