CGSC Strain#: 14161
Strain Designation: JM110      Source of Strain: J. Messing
Sex: F' Episome/Plasmid: F128-x
   Plasmid Markers/Mutations: lacIp-4000(lacI^Q), ΔlacZ58(M15), traD36, proA , proB
   Plasmid Comment: an F128-like F' found in Messing strains and their derivatives
Chromosomal Markers: thr-1, araC14, leuB6(Am), fhuA31, Δ(gpt-lac)0, tsx-78, glnX44(AS), galK2(Oc), galT22, λ^-, dcm-6, rpsL199(strR), dam-3, thiE1
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• dcm-6-- has a Gln to Arg mutation,also a TGG to TGA termination mutation,which results in dcm-vsr message degradation and causes a Vsr^- phenotype (Dar 1993).
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.
• M15 = alpha-complemented allele

References:

• Yanisch-Perron, C, J Vieira, J Messing 1985. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33(1):103-19.