CGSC Strain#: 3639
Strain Designation: CR34/454      Source of Strain: F. Bonhoeffer
Other Designations: PB33
Other Designations: AB3639
Sex: F- Chromosomal Markers: thr-1, leuB6(Am), fhuA21, lacY1, glnX44(AS), λ^-, rfbC1, thyA6, rpsL67(strR), thiE1, dnaB454(ts), deoC1
Strain Comments:

• Stops DNA synthesis immediately on shift to 43 degrees C.
• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• thyA6-- : Even with Luria or other complete media, supplement with thymine.
• thyA6-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• dnaB454(ts) was formerly called DNAts-454
• dnaB454(ts) was formerly called tsm-2
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• strR = streptomycin resist.
• ts = Temperature Sensitive

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