CGSC Strain#: 4014
Strain Designation: AB4014      Source of Strain:
Sex: F+ Chromosomal Markers: araBAD-1, thyA10, drm-5
Strain Comments:

• thyA10-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.

References:

• Vapnek, D, WD Rupp 1971. Identification of individual sex-factor DNA strands and their replication during conjugation in thermosensitive DNA mutants of Escherichia coli. J. Mol. Biol. 60(3):413-24.
• Vapnek, D, MB Lipman, WD Rupp 1971. Physical properties and mechanism of transfer of R factors in Escherichia coli. J. Bacteriol. 108(1):508-14.