CGSC Strain#: 4023
Strain Designation: AB3643 F⁺      Source of Strain:
Other Designations: AB4023
Sex: F- Chromosomal Markers: thr-1, leuB6(Am), lacY1, rfbC1, thyA6, rpsL67(strR), thiE1, dnaB43(ts)
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• thyA6-- : Even with Luria or other complete media, supplement with thymine.
• thyA6-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• strR = streptomycin resist.
• ts = Temperature Sensitive

References:

• Vapnek, D, WD Rupp 1971. Identification of individual sex-factor DNA strands and their replication during conjugation in thermosensitive DNA mutants of Escherichia coli. J. Mol. Biol. 60(3):413-24.