CGSC Strain#: 12518
Strain Designation: HMS174(DE3)      Source of Strain:
Sex: F- Chromosomal Markers: λDE3 [lacI, lacUV5-T7 gene 1, ind1, sam7, nin5], recA1, IN(rrnD-rrnE)1, rph-1, rpoB331(rifR), hsdR19
Strain Comments:

• λDE3 [lacI, lacUV5-T7 gene 1, ind1, sam7, nin5]-- Lambda DE3 has the phage T7 RNA polymerase under the control of the lacUV5 promoter inserted into the BamHI site of the Lambda cloning vector D69.
• recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
• IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• rifR = rifamycin resistance

References:

• Campbell, JL, CC Richardson, FW Studier 1978. Genetic recombination and complementation between bacteriophage T7 and cloned fragments of T7 DNA. Proc. Natl. Acad. Sci. U.S.A. 75(5):2276-80.