CGSC Strain#: 4046
Strain Designation: AB4046     
Source of Strain: M.G. Marinus
Sex: F+
Chromosomal Markers: thr-1,
araC14,
leuB6(Am)
,
Δ(gpt-proA)62,
lacY1,
tsx-33,
galK2(Oc)
,
hisG4(Oc)
,
thyA6,
thyA12,
rpsL31(strR)
or rpsL67(strR)
,
xylA5,
mtl-1,
thiE1,
dnaB43(ts)
Strain Comments: - leuB6(Am)-- Suppressed by serU suppressor (supH)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- thyA6-- : Even with Luria or other complete media, supplement with thymine.
- thyA6-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
- thyA12-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
- Δ(gpt-proA)62 was formerly called proA2
- galK2(Oc) was formerly called gal2
- thiE1 was formerly called thi-1
- Am = amber(UAG) mutation
- Oc = ochre(UAA) mutation
- strR = streptomycin resist.
- ts = Temperature Sensitive
References: - Bonhoeffer, F 1966. DNA transfer and DNA synthesis during bacterial conjugation. Z. Vererbungsl. 98(2):141-9.
- Vapnek, D, WD Rupp 1971. Identification of individual sex-factor DNA strands and their replication during conjugation in thermosensitive DNA mutants of Escherichia coli. J. Mol. Biol. 60(3):413-24.