CGSC Strain#: 4331
Strain Designation: KL105     
Source of Strain: M.C. Cappello
Sex: F-
Chromosomal Markers: thr-1,
araC14,
Δ(gpt-proA)62,
lacY1,
tsx-33,
glnX44(AS)
,
galK2(Oc)
,
λ-,
hisG4(Oc)
,
recA13,
rpsL31(strR)
,
xylA5,
mtl-1,
argE3(Oc)
,
thiE1Strain Comments: - This is a spontaneous leu+ revertant of AB2463.
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- recA13-- Amino acid substitution Leu 51 Phe
- Δ(gpt-proA)62 was formerly called proA2
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
- strR = streptomycin resist.
References: - Low, K.B. 1968. Proc.Natl.Acad.Sci.USA 60:161-0