CGSC Strain#: 4369
Strain Designation: KL198     
Source of Strain: M.C. Cappello
Sex: F-
Chromosomal Markers: galK35,
λ-,
pyrD34,
trpC45,
his-68,
thyA53,
rpsE2117(SpcR)
,
rpsL118(strR)
,
bgl-1,
ilvD16,
polA1(Am)
,
deoC2 or deo-44Strain Comments: - galK35-- is Brenner's gal-U99 from Hfr 3000 U99 and has been called galK99
- thyA53-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
- polA1(Am)-- normal levels exonuc.;premature term.of polymerase <1% WT level. MMSS,UVS, viable at all temps. Grindley MGG143
- deoC2 or deo-44-- deo-44 is a mutation in either deoC or deoB
- Am = amber(UAG) mutation
- SpcR = spectinomycin resistant
- strR = streptomycin resist.
References: - Lloyd, R.G., K.B. Low, G.N. Godson, E.A. Birge 1974. Isolation and characterization of an Escherichia coli K-12 mutant with a temperature-sensitive RecA- phenotype. J.Bacteriol. 120:407-415