CGSC Strain#: 14178
Strain Designation: RFM443      Source of Strain:
Sex: F- Chromosomal Markers: Δ(codB-lacI)3, galK2(Oc), λ^-, IN(rrnD-rrnE)1, rpsL200(strR), rph-1
Strain Comments:

• Δ(codB-lacI)3-- is a deletion of the entire lac operon, from the strain Hfr 3000 X74 of Jacob. Refs. on extent of deletion: Sung et al.1987,J.Bact.169:2639; Gerdes et al.2001
• Δ(codB-lacI)3-- Shown by Ferrandez et al. 1997 to extend to mhpD
• Δ(codB-lacI)3-- Shown by Durfee et al. to extend from codon 292 of yahG through mhpE
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• Δ(codB-lacI)3 was formerly called Δ(lac)3
• Δ(codB-lacI)3 was formerly called Δ(yahG-mhpE)
• Δ(codB-lacI)3 was formerly called X74
• Δ(codB-lacI)3 was formerly called DE(lac)6
• Δ(codB-lacI)3 was formerly called Δlac74
• galK2(Oc) was formerly called gal₂
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

References:

• Menzel, R 1989. A microtiter plate-based system for the semiautomated growth and assay of bacterial cells for beta-galactosidase activity. Anal. Biochem. 181(1):40-50.