CGSC Strain#: 4388
Strain Designation: DS68      Source of Strain: D.A. Steege
Sex: F- Chromosomal Markers: lacZ53(Am), Δ(galK-uvrB)45, LAMcI857(ts), trpA49(Am), serU132(AS), relA1, rpsL150(strR), arg-47
Strain Comments:

• LAMcI857 is integrated very close to serU(supD).
• lacZ53(Am)-- is the Paris lac^-_{Y14 amber}.
• trpA49(Am)-- is the amber mutation formerly called trp^-_8am by Brenner.
• rpsL150(strR)-- T to C transition mutation resulting in a K43R amino acid substitution.
• arg-47-- is a point mutation in the argC-H cluster
• serU132(AS) was formerly called Su1⁺
• serU132(AS) was formerly called supD32
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• strR = streptomycin resist.
• ts = Temperature Sensitive

References:

• Steege, DA, B Low 1975. Isolation and characterization of lambda transducing bacteriophages for the su1+ (supD minus) amber suppressor of Escherichia coli. J. Bacteriol. 122(1):120-8.