CGSC Strain#: 14182
Strain Designation: MG1655(motile)/pWTZ594     
Source of Strain: J.E. Wertz
Sex: F-
Episome/Plasmid: pWTZ594
Plasmid Markers/Mutations: bla(ApR)
,
lacZp-GFPuv(gfp),
ori[pMB1] Plasmid Comment: This plasmid was constructed by fusing the GFPuv gene behind the lacZ promoter in pBluescript KS+
Chromosomal Markers: λ-,
rph-1Strain Comments: - This is an isolate of MG1655 (CGSC#6300) that has been selected and verified for motility for those people requesting a motile WT strain.
- rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
- ApR = ampicillin resistant
- gfp = green fluorescent protein
References: