CGSC Strain#: 4598
Strain Designation: W945      Source of Strain: J. Lederberg
Sex: F- Chromosomal Markers: thr-1, araC14, leuB6(Am), lacY1, glnX44(AS), galK2(Oc), malT1(λ^R), xylA5, mtl-1, thiE1
Strain Comments:

• This should have been designated CGSC#4958. Only J. Cronan received this erroneous designation.
• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• LamR = Lambda-resistant
• Oc = ochre(UAA) mutation

References:

• Watson, J.D., W. Hayes 1953. Genetic exchange in Escherichia coli K-12; evidence for three linkage groups. Proc.Natl.Acad.Sci.USA 39:416-426