CGSC Strain#: 4915
Strain Designation: JP103      Source of Strain: A.J. Pittard
Sex: F' Episome/Plasmid: F61
   Plasmid Markers/Mutations: ilvC7, traD361(ts), ilvE , ilvD , ilvA , ilvC , PO13.
Chromosomal Markers: Δ(gpt-proA)62, lacY1 or lacZ4, glnX44(AS), galK2(Oc), hisG4(Oc), ilvC7, argE3(Oc), thiE1, tfr-3
Strain Comments:

• Transfers F61 at 32 degress C but not at 42 degrees C.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• Δ(gpt-proA)62 was formerly called proA2
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• ts = Temperature Sensitive

References:

• Walker, EM, J Pittard 1969. Temperature-sensitive conjugation-defective F factor in Escherichia coli. J. Bacteriol. 100(1):319-28.