CGSC Strain#: 5086
Strain Designation: T-3      Source of Strain: B. Magasanik
Sex: F- Chromosomal Markers: thr-1, leuB6(Am), glnX44(AS), λ^def, ptsG21, e14-, relA1, rpsL8, thiE1
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• ptsG21-- was formerly called cat-1 and, later, gpt-1.
• e14--- : Loss of the e14 element makes the strain McrA^-.
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor

References:

• Loomis, W.F., B. Magasanik 1965. Genetic control of catabolite repression of the lac operon in Escherichia coli. Biochem.Biophys.Res.Commun. 20:230-234
• Tyler, B, R Wishnow, WF Loomis, B Magasanik 1969. Catabolite repression gene of Escherichia coli. J. Bacteriol. 100(2):809-16.
• Curtis, S.J., W. Epstein 1975. Phosphorylation of D-glucose in Escherichia coli mutants defective in glucosephosphotransferase, mannosephosphotransferase, and glucokinase. J.Bacteriol. 122:1189-1199