CGSC Strain#: 5469
Strain Designation: AB1450      Source of Strain: A.J. Pittard
Other Designations: NIH#N185
Sex: F- Chromosomal Markers: fhuA2::IS2, lacY1 or lacZ4, tsx-7, glnX44(AS), gal-6, λ^-, hisG1, gltB13, rpsL8 or rplL9(L?) or rpsL17, malT1(λ^R), xyl-7, mtlA2, ilvD16, metB1, ΔargH1, thiE1
Strain Comments:

• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• hisG1-- This mutation has been reported as either a frameshift or a 36 nt deletion
• fhuA2::IS2 was formerly called fhuA2
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• xyl-7 was formerly called xylA7
• = strep-resistant
• ::IS2 = Insertion of IS2
• AS = amber(UAG) suppressor
• LamR = Lambda-resistant

References:

• PITTARD, J, JS LOUTIT, EA ADELBERG 1963. GENE TRANSFER BY F' STRAINS OF ESCHERICHIA COLI K-12. I. DELAY IN INITIATION OF CHROMOSOME TRANSFER. J. Bacteriol. 85:1394-401.
• Marsh, NJ, DE Duggan 1972. Ordering of mutant sites in the isoleucine-valine genes of Escherichia coli by use of merogenotes derived from F 14 : a new procedure for fine-structure mapping. J. Bacteriol. 109(2):730-40.
• Berberich, M.A. 1972. A glutamate-dependent phenotype in E. coli K-12: the result of two mutations. Biochem.Biophys.Res.Commun. 47:1498-1503