CGSC Strain#: 14197
Strain Designation: WTZ16002     
Source of Strain: J.E. Wertz
Sex: F-
Chromosomal Markers: ΔlacY884::FRT,
ΔompT774::kan,
λ-,
IN(rrnD-rrnE)1,
rph-1Strain Comments: - W3110 with lacY784::kan transduced from JW0334-1 and then the kan cassette removed
- ΔlacY884::FRT-- This is the ΔlacY784::kan allele with the kan cassette removed leaving an FRT scar
- ΔompT774::kan-- The primers used to make this knockout were TCACCTCGGGGAAATTTTAGTTGGCGTTCTTAAAATGTGTACTTAAGACCATTCCGGGGATCCGTCGACC and TAAAAAATACATATTCAATCATTAAAACGATTGAATGGAGAACTTTTATGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
- rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
- = kanamycin resistant
References: