CGSC Strain#: 14199
Strain Designation: WTZ17002     
Source of Strain: J.E. Wertz
Sex: F-
Chromosomal Markers: ΔfhuA866::FRT,
ΔlacY884::FRT,
ΔompT874::FRT,
λ-,
IN(rrnD-rrnE)1,
rph-1Strain Comments: - W3110 with lacY784, ompT874, and fhuA866 transduced from keio strains and then the kan cassette removed
- ΔlacY884::FRT-- This is the ΔlacY784::kan allele with the kan cassette removed leaving an FRT scar
- ΔompT874::FRT-- This is the ΔompT774::kan allele with the kan cassette removed leaving an FRT scar
- IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
- rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
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