CGSC Strain#: 13669
Strain Designation: pLC36-11     
Source of Strain: Sex: F+
Episome/Plasmid: pLC36-11A
Plasmid Markers/Mutations: thr-1,
araC14,
leuB6(Am)
,
lacY1,
glnX44(AS)
,
galK2(Oc)
,
λ-,
mtl-1,
thiE1,
galT22,
recA56,
xylA5,
ΔtrpE63,
flgD ,
flgE ,
flgF ,
flgG ,
flgH ,
flgI Plasmid Comment: Mixed clone. Hybridizes with Kohara clones 233, 234, 235 and carries {\it flgDEFGHI} at ca. 24.35 min., and also hybridizes with Kohara clones 519 and 658. See also pLC36-11B and pLC36-11C
Chromosomal Markers: thr-1,
araC14,
leuB6(Am)
,
lacY1,
glnX44(AS)
,
galK2(Oc)
,
galT22,
λ-,
ΔtrpE63,
recA56,
xylA5,
mtl-1,
thiE1Strain Comments: - leuB6(Am)-- Suppressed by serU suppressor (supH)
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- recA56-- Amino acid substitution Arg 60 Cys
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- ΔtrpE63 was formerly called DE(trpE5)
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
References: - Clarke, L, J Carbon 1976. A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome. Cell 9(1):91-9.
- Komeda, Y., M. Silverman, M.(I.) Simon 1978. Identification of the structural gene for the hook subunit protein of Escherichia coli flagella. J.Bacteriol. 133:364-371
- Komeda, Y., M. Silverman, P. Matsumura, M.(I.) Simon 1978. Genes for the hook-basal body proteins of the flagellar apparatus in Escherichia coli. J.Bacteriol. 134:655-667
- Nishimura, A., K. Akiyama, Y. Kohara, K. Horiuchi 1992. Correlation of a subset of the pLC plasmids to the physical map of Escherichia coli K-12. Microbiol.Rev. 56:137-151