CGSC Strain#: 13677
Strain Designation: pLC44-14     
Source of Strain: Sex: F+
Episome/Plasmid: pLC44-14
Plasmid Markers/Mutations: thr-1,
araC14,
leuB6(Am)
,
lacY1,
glnX44(AS)
,
galK2(Oc)
,
λ-,
mtl-1,
thiE1,
galT22,
recA56,
xylA5,
ΔtrpE63,
ubiA ,
lexA ,
dnaB ,
plsB ,
dinF ,
alr ,
tyrB ,
tnm ,
ubiC ,
uvrA ,
dgkA Plasmid Comment: Hybridizes with Kohara clones 634-636. Genes above attributed to plasmid by Phillips et al. and refs below.
Chromosomal Markers: thr-1,
araC14,
leuB6(Am)
,
lacY1,
glnX44(AS)
,
galK2(Oc)
,
galT22,
λ-,
ΔtrpE63,
recA56,
xylA5,
mtl-1,
thiE1Strain Comments: - leuB6(Am)-- Suppressed by serU suppressor (supH)
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- recA56-- Amino acid substitution Arg 60 Cys
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- ΔtrpE63 was formerly called DE(trpE5)
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
References: - Clarke, L, J Carbon 1976. A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome. Cell 9(1):91-9.
- Brent, R., M. Ptashne 1980. The lexA gene product represses its own promoter. Proc.Natl.Acad.Sci.USA 77:1932-1936
- Little, R. 1980. Isolation of recombinant plasmids and phage carrying the lexA gene of Eschericia coli K-12. Gene 10:237-247
- Mount, DW, JW Little, SH Edmiston 1980. Influence of plasmids carrying the lexA gene on DNA repair and related processes in Escherichia coli K-12. Mol. Gen. Genet. 177(3):477-83.
- Lightner, V.A., T.J. Larson, P. Tailleur, G.D. Kantor, C.H.R. Raetz, R.M. Bell, P. Modrich 1980. Membrane phospholipid synthesis in Escherichia coli. Cloning of a structural gene (plsB) of the sn-glycerol-3-phosphate acyltransferase. J.Biol.Chem. 255:
- Miki, T., Y. Ebina, F. Kishi, A. Nakazawa 1981. Organization of the lexA gene of Escherichia coli and nucleotide sequence of the regulatory region. Nucleic Acids Res. 9:529-543
- Neidhardt, F.C., V. Vaughn, T.A. Phillips, P.L. Bloch 1983. Gene-protein index of Escherichia coli K-12. Microbiol.Rev. 47:231-284
- Nishimura, A., K. Akiyama, Y. Kohara, K. Horiuchi 1992. Correlation of a subset of the pLC plasmids to the physical map of Escherichia coli K-12. Microbiol.Rev. 56:137-151