CGSC Strain#: 2553
Strain Designation: AB2553     
Source of Strain: G. Eggertsson
Sex: F-
Chromosomal Markers: araBAD-9,
fhuA1,
Δ(gpt-proA)62,
lacY1,
lacZ4,
tsx-3,
glnX44(AS)
,
galK2(Oc)
,
trpE3(Oc)
,
hisG4(Oc)
,
rpsL8,
malT1(λ
R)
,
mtl-1,
metE46,
tyrU20(OS)
,
rplL9(L?)
,
thiE1,
ilv-1Strain Comments: - glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- tyrU20(OS)-- polar; DES. Use AB2577 for tyrU20. tyrosine tRNA2
- Δ(gpt-proA)62 was formerly called proA2
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- AS = amber(UAG) suppressor
- L? = assignment uncertain
- LamR = Lambda-resistant
- Oc = ochre(UAA) mutation
- OS = ochre(UAA) suppressor
References: