CGSC Strain#: 4435
Strain Designation: ES365      Source of Strain: E. Siegel
Sex: F- Chromosomal Markers: araC14, leuB6(Am), secA206(aziR), fhuA23, lacY1, tsx-67, lon-9, purE42, glnX44(AS), galK2(Oc), trpE38, rpsL109(strR), xylA5, mtlA2, thiE1, his61
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• lon-9-- = capR9 of Markovitz.
• purE42-- :Strains with this marker are derivatives of Χ148 and may carry malA38LAMR and a suppressor of the Mal^- phenotype, sup-78,near his.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• aziR = azide resistance
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

References:

• Radke, K.L., E.C. Siegel 1971. Mutation preventing capsular polysaccharide synthesis in Escherichia coli K-12 and its effect on bacteriophage resistance. J.Bacteriol. 106:432-437