CGSC Strain#: 5733
Strain Designation: W945T1-2,his      Source of Strain: U. Henning
Sex: F- Chromosomal Markers: thr-1, araC14, leuB6(Am), lacY1, glnX44(AS), galK2(Oc), trp-59, mdh-2, rpsL264(strR), xylA5, mtl-1, thiE1, his216
Strain Comments:

• Strain should carry malA1, LAMR but is Mal⁺.
• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• mdh-2-- It has been noted that mdh mutants in general are leaky and are distinguished from wild-type alleles by colony size at 24-46 hours on media containing malate (autoclaved and at pH 6.0).
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

References:

• Courtright, J.B., U. Henning 1970. Malate dehydrogenase mutants in Escherichia coli K-12. J.Bacteriol. 102:722-728