CGSC Strain#: 6873
Strain Designation: SR776     
Source of Strain: K.C. Smith
Sex: F-
Chromosomal Markers: araC14,
leuB6(Am)
,
lacY1,
tsx-33,
qsr'-0,
glnX44(AS)
,
galK2(Oc)
,
hisG4(Oc)
,
rfbC1,
mgl-51,
rpsL31(strR)
,
kdgK51,
xylA5,
mtl-1,
argE3(Oc)
,
thiE1,
radA100Strain Comments: - leuB6(Am)-- Suppressed by serU suppressor (supH)
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- rfbC1 was formerly called rfbD1
- thiE1 was formerly called thi-1
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
- strR = streptomycin resist.
References: - Diver, W.P., N.J. Sargentini, K.C. Smith 1982. A mutation (radA100) in Escherichia coli that selectively sensitizes cells grown in rich medium to x- or u.v.-radiation, or methyl methanesulphonate. Int.J.Radiat.Biol. 42:339-346
- Diver, W.P., N.J. Sargentini, K.C. Smith 1982. A mutation (radA100) in Escherichia coli that selectively sensitizes cells grown in rich medium to x- or u.v.-radiation, or methyl methanesulphonate. Int.J.Radiat.Biol. 42:339-346