CGSC Strain#: 6876
Strain Designation: SR1060
Source of Strain: K.C. Smith
Sex: F-
Chromosomal Markers: leuB19,
lacZ53(Am)
,
bioA2,
recN2001,
thyA36,
rpsL151(strR)
,
metE70,
rha-5,
malB45,
deoC2Strain Comments: - lacZ53(Am)-- is the Paris lac-Y14 amber.
- thyA36-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
- Am = amber(UAG) mutation
- strR = streptomycin resist.
References: - Sargentini, N.J., K.C. Smith 1983. Characterization of an Escherichia coli mutant (radB101) sensitive to gamma and uv radiation, and methyl methanesulfonate. Radiat.Res. 93:461-478
- Sargentini, N.J., K.C. Smith 1983. Characterization of an Escherichia coli mutant (radB101) sensitive to gamma and uv radiation, and methyl methanesulfonate. Radiat.Res. 93:461-478