CGSC Strain#: 6875
Strain Designation: SR1059      Source of Strain: K.C. Smith
Sex: F- Chromosomal Markers: leuB19, lacZ53(Am), bioA2, thyA36, rpsL151(strR), metE70, rha-5, malB45, deoC2
Strain Comments:

• lacZ53(Am)-- is the Paris lac^-_{Y14 amber}.
• thyA36-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• Am = amber(UAG) mutation
• strR = streptomycin resist.

References:

• Sargentini, N.J., K.C. Smith 1983. Characterization of an Escherichia coli mutant (radB101) sensitive to gamma and uv radiation, and methyl methanesulfonate. Radiat.Res. 93:461-478
• Sargentini, N.J., K.C. Smith 1983. Characterization of an Escherichia coli mutant (radB101) sensitive to gamma and uv radiation, and methyl methanesulfonate. Radiat.Res. 93:461-478