CGSC Strain#: 7105
Strain Designation: SL101      Source of Strain: J. Speyer
Sex: F- Chromosomal Markers: Δ(gpt-proA)62, lacY1, glnX44(AS), galK2(Oc), λ^-, purB93(ts), rfbC1, rpsL35(strR), xylA5, mtl-1, thiE1, rac-
Strain Comments:

• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• purB93(ts)-- has a Ts requirement for adenine and an antimutator phenotype (resistance to MNNG and other alkylating agents).
• Δ(gpt-proA)62 was formerly called proA2
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• purB93(ts) was formerly called mud
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.
• ts = Temperature Sensitive

References:

• Lyons, S., J. Speyer, P.F. Schendel 1985. Interaction of an antimutator gene with DNA repair pathways in Escherichia coli K-12. Mol.Gen.Genet. 198:336-347