CGSC Strain#: 7122
Strain Designation: SN164      Source of Strain: H. Nakayama
Sex: F- Chromosomal Markers: leuB6(Am), fhuA2::IS2, lacY1, tsx-1, glnX44(AS), gal-6, λ^-, trp-31, hisG1, rfbC1, galP63, argG6, rpsL104, malT1(λ^R), xyl-7, mtlA2, metB1, thiA-46(ts)
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• hisG1-- This mutation has been reported as either a frameshift or a 36 nt deletion
• fhuA2::IS2 was formerly called fhuA2
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• rfbC1 was formerly called rfbD1
• xyl-7 was formerly called xylA7
• = strep-resistant
• ::IS2 = Insertion of IS2
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• LamR = Lambda-resistant
• ts = Temperature Sensitive

References:

• Imamura, N., H. Nakayama 1982. thiK and thiL loci of Escherichia coli. (Erratum: 152:1308,1982). J.Bacteriol. 151:708-717
• Mizote, T., H. Nakayama 1989. The thiM locus and its relation to phosphorylation of hydroxyethylthiazole in Escherichia coli. J.Bacteriol. 171:3228-3232