CGSC Strain#: 13885
Strain Designation: pLC22-11     
Source of Strain: Sex: F+
Episome/Plasmid: pLC22-11
Plasmid Markers/Mutations: thr-1,
araC14,
leuB6(Am)
,
lacY1,
glnX44(AS)
,
galK2(Oc)
,
λ-,
mtl-1,
thiE1,
galT22,
recA56,
xylA5,
ΔtrpE63,
rrnD ,
rrfD ,
ileU ,
alaU ,
rrsD ,
aroE Plasmid Comment: May be a mixed clone. Hybridizes with Kohara clones 522-523 ca. 71.5 and carries rrnD, rrfD, ileU, alaU, rrsD, and aroE between 73.7 and 73.9.
Chromosomal Markers: thr-1,
araC14,
leuB6(Am)
,
lacY1,
glnX44(AS)
,
galK2(Oc)
,
galT22,
λ-,
ΔtrpE63,
recA56,
xylA5,
mtl-1,
thiE1Strain Comments: - leuB6(Am)-- Suppressed by serU suppressor (supH)
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- recA56-- Amino acid substitution Arg 60 Cys
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- ΔtrpE63 was formerly called DE(trpE5)
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
References: - Clarke, L, J Carbon 1976. A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome. Cell 9(1):91-9.
- Ikemura, T, M Nomura 1977. Expression of spacer tRNA genes in ribosomal RNA transcription units carried by hybrid Col E1 plasmids in E. coli. Cell 11(4):779-93.
- Kenerley, M.E., E.A. Morgan, L. Post, L. Lindahl, M. Nomura 1977. Characterization of hybrid plasmids carrying individual ribosomal ribonucleic acid transcription units of Escherichia coli. J.Bacteriol. 132:931-949
- Clarke, L., J. Carbon 1979. Selection of specific clones from colony banks by suppression or complementation tests. Methods Enzymol. 68:396-408
- Ellwood, M. Nomura 1982. Chromosomal locations of the genes for rRNA in Escherichia coli K-12. J.Bacteriol. 149:458-468
- Nishimura, A., K. Akiyama, Y. Kohara, K. Horiuchi 1992. Correlation of a subset of the pLC plasmids to the physical map of Escherichia coli K-12. Microbiol.Rev. 56:137-151