CGSC Strain#: 7265
Strain Designation: GE1377     
Source of Strain: G. Eggertsson
Sex: Hfr
Point of Origin#: 12
Map Position: 68.80
Direction of Marker Transfer: Clockwise
Chromosomal Markers: thr-1,
leuB6(Am)
,
panD2,
hemL207,
lacZ4,
glnX44(AS)
,
λ-,
rfbC1,
rpsL8,
thiE1Strain Comments: - All hemL mutations are leaky. The mutants grow in the absence of a-aminolevulinic acid but grow better in the presence of this compound. (25 ug/ml)
- leuB6(Am)-- Suppressed by serU suppressor (supH)
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- rfbC1 was formerly called rfbD1
- thiE1 was formerly called thi-1
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
References: - Ilag, L.L., D. Jahn, G. Eggertsson, D. Soll 1991. The Escherichia coli hemL gene encodes glutamate 1-semialdehyde aminotransferase. J.Bacteriol. 173:3408-3413