CGSC Strain#: 7079
Strain Designation: RS2      Source of Strain: R. Sternglanz
Sex: F- Chromosomal Markers: gal-25, λ^-, topA10, pyrF287, fnr-1, rpsL195(strR), iclR7(Const), trpR72(Am)
Strain Comments:

• pyrF287-- marker comes from Hfr 3000 YA287 and is described as a stable allele.
• iclR7(Const)-- renders this strain constitutive for isocitrate lyase.
• fnr-1 was formerly called nirR1
• Am = amber(UAG) mutation
• Const = constitutive
• strR = streptomycin resist.

References:

• Sternglanz, R., S. DiNardo, K.A. Voelkel, Y. Nishimura, Y. Hirota, K. Becherer, L. Zumstein, J.C. Wang 1981. Mutations in the gene coding for Escherichia coli DNA topoisomerase I affect transcription and translation. Proc.Natl.Acad.Sci.USA 78:2747-2751
• DiNardo, S., K.A. Voelkel, R. Sternglanz, A.E. Reynolds, A. Wright 1982. Escherichia coli DNA topoisomerase I mutants have compensatory mutations in DNA gyrase genes. Cell 31:43-51