CGSC Strain#: 7089
Strain Designation: DH1/pCD100      Source of Strain: C.R.H. Raetz
Sex: F- Episome/Plasmid: pCD100
   Plasmid Markers/Mutations: cdsA
Chromosomal Markers: glnX44(AS), λ^-, rfbC1, gyrA96(NalR), recA1, endA1, spoT1, thiE1, hsdR17
Strain Comments:

• pCD100 carries the cds gene.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• gyrA96(NalR)-- resistant to 200ug/ml nalidixic acid
• gyrA96(NalR)-- GAC to AAC at codon 87
• recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
• endA1-- G to A transition mutation resulting in E208K amino acid substitution.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• gyrA96(NalR) was formerly called
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• = good miniprep dsDNA quality
• AS = amber(UAG) suppressor
• NalR = Nalidix.acidResist.

References:

• Icho, T, CE Bulawa, CR Raetz 1985. Molecular cloning and sequencing of the gene for CDP-diglyceride hydrolase of Escherichia coli. J. Biol. Chem. 260(22):12092-8.
• Icho, T., C.P. Sparrow, C.H.R. Raetz 1985. Molecular cloning and sequencing of the gene for CDP-diglyceride synthetase of Escherichia coli. J.Biol.Chem. 260:12078-12083