CGSC Strain#: 7257
Strain Designation: MEC1      Source of Strain: M.A. Eisenberg
Sex: F- Chromosomal Markers: thr-1, leuB6(Am), glnX44(AS), λ^-, bioA109, rfbC1, thiE1
Strain Comments:

• We have not yet verified this genotype. It is based on a published citation of Y10-1 as the parent strain.
• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor

References:

• Rolfe, B., M.A. Eisenberg 1968. Genetic and biochemical analysis of the biotin loci of Escherichia coli K-12. J.Bacteriol. 96:515-524