CGSC Strain#: 7286
Strain Designation: JC14604      Source of Strain: A.J. Clark
Sex: F- Chromosomal Markers: araC14, Δ(lacY-lacZ)286, tsx-33, glnX44(AS), galK2(Oc), λ^-, lacZ95=phi80dIIlacZ9, hisG4(Oc), Δ(recA-srl)306, srlR301::Tn10, rpsL31(strR), xylA5, mtl-1, argE3(Oc), thiE1, hsdR4
Strain Comments:

• Δ(lacY-lacZ)286-- is a deletion of part of lacY and part of lacZ which has been called lacMS286.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• lacZ95=phi80dIIlacZ9-- is an intragenic deletion of lacZ which does not overlap DE286, carried on φ80. (Konrad, 1977, J.Bacteriol.130:167)
• Δ(recA-srl)306-- removes all, or nearly all, of the recA gene and extends into srlR, but does not remove the Tn10 insertion in srlR
• Δ(lacY-lacZ)286 was formerly called lacMS286
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• lacZ95=phi80dIIlacZ9 was formerly called lacBK1
• thiE1 was formerly called thi-1
• = tetracycline resistant
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

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