CGSC Strain#: 7287
Strain Designation: ER11      Source of Strain: R.E. Moses
Sex: F- Chromosomal Markers: thr-1, leuB6(Am), fhuA21, dnaE486(ts), lacY1, glnX44(AS), λ^-, e14-, rfbC1, thyA6, rpsL67(strR), glpR200(glp^c), zid-2086::Tn10, thiE1, deoC1, met-89
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• e14--- : Loss of the e14 element makes the strain McrA^-.
• thyA6-- : Even with Luria or other complete media, supplement with thymine.
• thyA6-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• zid-2086::Tn10-- near gyrB
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• = tetracycline resistant
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• glp^c = constitutive expr.glp regulon
• strR = streptomycin resist.
• ts = Temperature Sensitive

References:

• Bryan, S.K., R.E. Moses 1984. Map location of the pcbA mutation and physiology of the mutant. J.Bacteriol. 158:216-221