CGSC Strain#: 7288
Strain Designation: SK707      Source of Strain: S.R. Kushner
Sex: F- Chromosomal Markers: Δ(lacY-lacZ)286, tsx-3, glnX44(AS), galK2(Oc), λ^-, lacZ95=phi80dIIlacZ9, hisG4(Oc), rfbC1, rpsL281(strR), xyl-7, mtl-1, ilvD188(Oc), metE46, ΔargH1, thiE1
Strain Comments:

• Δ(lacY-lacZ)286-- is a deletion of part of lacY and part of lacZ which has been called lacMS286.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• lacZ95=phi80dIIlacZ9-- is an intragenic deletion of lacZ which does not overlap DE286, carried on φ80. (Konrad, 1977, J.Bacteriol.130:167)
• Δ(lacY-lacZ)286 was formerly called lacMS286
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• lacZ95=phi80dIIlacZ9 was formerly called lacBK1
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• xyl-7 was formerly called xylA7
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

References:

• Washburn, B.K., S.R. Kushner 1991. Construction and analysis of deletions in the structural gene (uvrD) for DNA helicase II of Escherichia coli. J.Bacteriol. 173:2569-2575