CGSC Strain#: 7288
Strain Designation: SK707     
Source of Strain: S.R. Kushner
Sex: F-
Chromosomal Markers: Δ(lacY-lacZ)286,
tsx-3,
glnX44(AS)
,
galK2(Oc)
,
λ-,
lacZ95=phi80dIIlacZ9,
hisG4(Oc)
,
rfbC1,
rpsL281(strR)
,
xyl-7,
mtl-1,
ilvD188(Oc)
,
metE46,
ΔargH1,
thiE1Strain Comments: - Δ(lacY-lacZ)286-- is a deletion of part of lacY and part of lacZ which has been called lacMS286.
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- lacZ95=phi80dIIlacZ9-- is an intragenic deletion of lacZ which does not overlap DE286, carried on φ80. (Konrad, 1977, J.Bacteriol.130:167)
- Δ(lacY-lacZ)286 was formerly called lacMS286
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- lacZ95=phi80dIIlacZ9 was formerly called lacBK1
- rfbC1 was formerly called rfbD1
- thiE1 was formerly called thi-1
- xyl-7 was formerly called xylA7
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
- strR = streptomycin resist.
References: - Washburn, B.K., S.R. Kushner 1991. Construction and analysis of deletions in the structural gene (uvrD) for DNA helicase II of Escherichia coli. J.Bacteriol. 173:2569-2575