CGSC Strain#: 7383
Strain Designation: CAG18462A     
Source of Strain: C.A. Gross
Sex: F-
Chromosomal Markers: λ-,
rph-1,
chpS603::Tn10Strain Comments: - The Tn10 in this strain did not segregate with the tus marker (see mutation zdh-603) and we have removed it from this strain, now designated CAG18462A.
- rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
- chpS603::Tn10-- The insertion originally reported in this strain was located at ca. 37.1 min. ( zdh-603::Tn10). PCR analysis by Nichols et al. placed it at nucleotide 4,446,108 (95.837 min.)
- = tetracycline resistant
References: - Singer, M., T.A. Baker, G. Schnitzler, S.M. Deischel, M. Goel, W.F. Dove, K.J. Jaacks, A.D. Grossman, J.. Erickson, C.A. Gross 1989. A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of
- Nichols, BP, O Shafiq, V Meiners 1998. Sequence analysis of Tn10 insertion sites in a collection of Escherichia coli strains used for genetic mapping and strain construction. J. Bacteriol. 180(23):6408-11.