CGSC Strain#: 7460
Strain Designation: CAG18501     
Source of Strain: C.A. Gross
Sex: F-
Chromosomal Markers: λ-,
rph-1,
rbsD296::Tn10Strain Comments: - rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
- rbsD296::Tn10-- cotransduces 68% with ilv, counterclockwise. Shown by PCR analysis to be within the rbsD gene at nucleotide 3,931,475 (84.744 min.)
- rbsD296::Tn10 was formerly called zie-296::Tn10
- = tetracycline resistant
References: - Singer, M., T.A. Baker, G. Schnitzler, S.M. Deischel, M. Goel, W.F. Dove, K.J. Jaacks, A.D. Grossman, J.. Erickson, C.A. Gross 1989. A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of