CGSC Strain#: 7484
Strain Designation: CAG12019     
Source of Strain: C.A. Gross
Sex: F-
Chromosomal Markers: λ-,
rph-1,
zjf-920::Tn10Strain Comments: - rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
- zjf-920::Tn10-- cotransduces ca. 10% with fdp ( fbp) and 26% with pyrB. Shown by Nichols et al. to lie within orf yjcS, at nucleotide 4,303,986 (min. 95.757)
- zjf-920::Tn10 was formerly called zjg-920::Tn10
- = tetracycline resistant
References: - Singer, M., T.A. Baker, G. Schnitzler, S.M. Deischel, M. Goel, W.F. Dove, K.J. Jaacks, A.D. Grossman, J.. Erickson, C.A. Gross 1989. A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of
- Nichols, BP, O Shafiq, V Meiners 1998. Sequence analysis of Tn10 insertion sites in a collection of Escherichia coli strains used for genetic mapping and strain construction. J. Bacteriol. 180(23):6408-11.