CGSC Strain#: 6220
Strain Designation: KL286      Source of Strain: M.C. Cappello
Sex: F- Chromosomal Markers: garB10, fhuA22, phoA4(Am), galK35, serS14(ts), serC13, ompF627(T2R), trpC45, his-68, fadL701(T2R), relA1, rpsL118(strR), malT1(λ^R), pitA10, xyl-7, mtlA2, spoT1, rrnB-2, mcrB1, creC510
Strain Comments:

• phoA4(Am)-- is Garen's phoAS26 . It is suppressed by leuX151, glnV33(=supE33) and serU132(=supD32).
• galK35-- is Brenner's gal^-_U99 from Hfr 3000 U99 and has been called galK99
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• rrnB-2-- : loss of spacer loop sequence
• creC510-- The constitutive phenotype is due to an R77P amino acid substitution
• creC510 was formerly called phoM510
• xyl-7 was formerly called xylA7
• Am = amber(UAG) mutation
• Const = constitutive
• LamR = Lambda-resistant
• strR = streptomycin resist.
• T2R = T2 Resistant
• ts = Temperature Sensitive

References:

• Clarke, S.J., K.B. Low, W. Konigsberg 1973. Close linkage of the genes serC (for phosphohydroxy pyruvate transaminase) and serS (for seryl-transfer ribonucleic acid synthetase) in Escherichia coli K-12. J.Bacteriol. 113:1091-1095