CGSC Strain#: 6218
Strain Designation: KL788      Source of Strain: M.C. Cappello
Sex: F- Chromosomal Markers: Δ(gpt-lac)5, tsx-35, λ^-, sulA3, e14-, Rac-0, rfbC1, mgl-51, recA441(ts), relA1, rpsL31(strR), kdgK51, mtl-1, spoT1, thiE1, lexA71::Tn5, creC510
Strain Comments:

• Δ(gpt-lac)5-- is the deletion Δ( pro-lac)_X111 of Jacob and originated in the strain Hfr 3000 X111.
• e14--- : Loss of the e14 element makes the strain McrA^-.
• recA441(ts)-- formerly tif-1 (temp.-induced filamentation). Not a Rec^- phenotype, "constitutive protease activity" not requiring ss-DNA binding at hi temp.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• creC510-- The constitutive phenotype is due to an R77P amino acid substitution
• creC510 was formerly called phoM510
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• = kanamycin resistant
• Const = constitutive
• strR = streptomycin resist.
• ts = Temperature Sensitive

References:

• Krueger, J.H., S.J. Elledge, G.C. Walker 1983. Isolation and characterization of Tn5 insertion mutations in the lexA gene of Escherichia coli. J.Bacteriol. 153:1368-1378
• Miller, J.H., K.B. Low 1984. Specificity of mutagenesis resulting from the induction of the SOS system in the absence of mutagenic treatment. Cell 37:675-682