CGSC Strain#: 6856
Strain Designation: HT414      Source of Strain: H. Tabor
Sex: F- Chromosomal Markers: thr-1, fhuA21, lacY1, glnX44(AS), λ^-, Δ(speB-speA)97, Δ(speC-glcB)63, rplL9(L?), thiE1, hsdS1
Strain Comments:

• The rpsL allele is important; it makes this strain the only good Spe^- strain.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• AS = amber(UAG) suppressor
• L? = assignment uncertain

References:

• Tabor, H., C.W. Tabor, M.S. Cohn, E.W. Hafner 1981. Streptomycin resistance (rpsL) produces an absolute requirement for polyamines for growth of an Escherichia coli strain unable to synthesize putrescine and spermidine [delta(speA-speB) delta specC]. J