CGSC Strain#: 7528
Strain Designation: CAG18577gyrA
+     
Source of Strain: S. McAteer
Sex: F-
Chromosomal Markers: λ-,
atoS3142::Tn10kan,
rph-1Strain Comments: - CAG18577 was found to be gyrA- and S.McAteer constructed this gyrA+ strain from CAG18577.
- atoS3142::Tn10kan-- derived from atoS298::Tn10, formerly called zej-298::Tn10, shown by PCR to be within atoS at nucleotide 2,318,294(49.97 min.).
- atoS3142::Tn10kan-- : Cotransduction values for atoS298::Tn10 are 77% with ompC and 30% with gyrA.
- rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
- atoS3142::Tn10kan was formerly called zej-3142::Tn10kan
- = kanamycin resistant
References: