CGSC Strain#: 7531
Strain Designation: WL81300      Source of Strain: A. Bock
Sex: F- Chromosomal Markers: [araD139]_B/r, Δ(argF-lac)169, λ^-, flhD5301, Δ(fruK-yeiR)725(fruA25), Δ(recA-srl)306, relA1, ΔselB300::kan, rbsR22, Δ(fimB-fimE)632(::IS1), deoC1, spc-13
Strain Comments:

• [araD139]_B/r-- The thr-leu region was transduced from an E. coli B/r strain (SB3118) in early steps of strain derivation; ara or in some strains ara-leu or thr-leu are from B.
• Δ(argF-lac)169-- extends from mmuP through orfs preceding argF, through lac to mhpD, literally Δ(mmuP-mhpD)169. (Peters et al. 2003 JB 185:2017)
• Δ(argF-lac)169-- from strain Hfr3000 U169 was initially called ΔlacU169 and described as a lacZY mutation until found to include argF and lacI.
• flhD5301-- flagellar defect shown in Komeda and Iino
• Δ(fruK-yeiR)725(fruA25)-- : this fruA25 mutation deletes 29 codons of fruK, fruB and fruBKAp promoter to orf yeiR Peters et al. J.Bact.185:2017.
• Δ(recA-srl)306-- removes all, or nearly all, of the recA gene and extends into srlR, but does not remove the Tn10 insertion in srlR
• ΔselB300::kan-- : A fragment of the selB gene was replaced with the kan gene of Tn903
• Δ(fruK-yeiR)725(fruA25) was formerly called fruA25
• = kanamycin resistant
• ::IS1 = insertion of IS1
• fruA25 = former designation

References:

• Tormay, P., A. Sawers, A. Bock 1996. Role of stoichiometry between mRNA, translation factor SelB and selenocysteyl-tRNA in selenoprotein synthesis. Mol.Microbiol. 21:1253-1259
• Tormay, P., A. Sawers, A. Bock 1996. Role of stoichiometry between mRNA, translation factor SelB and selenocysteyl-tRNA in selenoprotein synthesis. Mol.Microbiol. 21:1253-1259