CGSC Strain#: 7532
Strain Designation: WL400     
Source of Strain: A. Bock
Sex: F-
Chromosomal Markers: [araD139]B/r,
Δ(argF-lac)169,
λ-,
selD204(CamR)
::cat,
flhD5301,
Δ(fruK-yeiR)725(fruA25),
relA1,
rpsL150(strR),
rbsR22,
Δ(fimB-fimE)632(::IS1),
deoC1Strain Comments: - [araD139]B/r-- The thr-leu region was transduced from an E. coli B/r strain (SB3118) in early steps of strain derivation; ara or in some strains ara-leu or thr-leu are from B.
- Δ(argF-lac)169-- extends from mmuP through orfs preceding argF, through lac to mhpD, literally Δ(mmuP-mhpD)169. (Peters et al. 2003 JB 185:2017)
- Δ(argF-lac)169-- from strain Hfr3000 U169 was initially called ΔlacU169 and described as a lacZY mutation until found to include argF and lacI.
- selD204(CamR)::cat-- inactivates selD gene by inserting cat gene from plasmid pACYC184 on a HinPI into the ClaI site
- flhD5301-- flagellar defect shown in Komeda and Iino
- Δ(fruK-yeiR)725(fruA25)-- : this fruA25 mutation deletes 29 codons of fruK, fruB and fruBKAp promoter to orf yeiR Peters et al. J.Bact.185:2017.
- rpsL150(strR)-- T to C transition mutation resulting in a K43R amino acid substitution.
- Δ(fruK-yeiR)725(fruA25) was formerly called fruA25
- = chloramphenicol resistant
- ::IS1 = insertion of IS1
- CamR = chloramphenicol resistant
- fruA25 = former designation
- strR = streptomycin resist.
References: - Leinfelder, W., K. Forchhammer, B. Veprek, E. Zehelein, A. Bock 1990. In vitro synthesis of selenocysteinyl-tRNA(UCA) from seryl-tRNA(UCA): involvement and characterization of the selD gene product. Proc.Natl.Acad.Sci.USA 87:543-547
- Leinfelder, W., K. Forchhammer, B. Veprek, E. Zehelein, A. Bock 1990. In vitro synthesis of selenocysteinyl-tRNA(UCA) from seryl-tRNA(UCA): involvement and characterization of the selD gene product. Proc.Natl.Acad.Sci.USA 87:543-547