CGSC Strain#: 7514
Strain Designation: CAG12205      Source of Strain: C.A. Gross
Sex: Hfr    Point of Origin#: 13   Map Position: 84.98   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: leuB6(Am), lacY1 or lacZ4, glnX44(AS), gal-6, λ^-, rfbC1, aer-3159::Tn10kan, thiE1
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• aer-3159::Tn10kan-- : derived from aer-3075::Tn10, shown by PCR (Nichols et al.1998) to be at nucleotide 3,215,934 (69.321 in.)
• aer-3159::Tn10kan was formerly called zgi-3159::Tn10kan
• aer-3159::Tn10kan was formerly called zgj-3159::Tn10kan
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• = kanamycin resistant
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor

References:

• Singer, M., T.A. Baker, G. Schnitzler, S.M. Deischel, M. Goel, W.F. Dove, K.J. Jaacks, A.D. Grossman, J.. Erickson, C.A. Gross 1989. A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of
• Nichols, BP, O Shafiq, V Meiners 1998. Sequence analysis of Tn10 insertion sites in a collection of Escherichia coli strains used for genetic mapping and strain construction. J. Bacteriol. 180(23):6408-11.