CGSC Strain#: 4309
Strain Designation: KL725     
Source of Strain: M.C. Cappello
Other Designations: KLF6
Sex: F'
Episome/Plasmid: F106
Plasmid Markers/Mutations: Chromosomal Markers: galK35,
λ-,
pyrD34,
trpC45,
his-68,
recA1,
rpsL118(strR)
,
malT1(λ
R)
,
xyl-7,
mtlA2Strain Comments: - This strain must be maintained on medium containing trp, his, thi and lacking ura.
- galK35-- is Brenner's gal-U99 from Hfr 3000 U99 and has been called galK99
- recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
- xyl-7 was formerly called xylA7
- LamR = Lambda-resistant
- strR = streptomycin resist.
References: - Low, K.B. 1968. Formation of merodiploids in matings with a class of Rec- recipient strains of Escherichia coli K12. Proc.Natl.Acad.Sci.USA 60:160-167