CGSC Strain#: 5028
Strain Designation: E508     
Source of Strain: Wechsler and Gross
Sex: F-
Chromosomal Markers: thr-1,
leuB6(Am)
,
fhuA21,
lacY1,
glnX44(AS)
,
λ-,
rfbC1,
thyA6,
rpsL67(strR)
,
glpR101,
dnaA508(ts)
,
thiE1,
deoC1Strain Comments: - dnaA508 is a temperature-sensitive mutation.
- leuB6(Am)-- Suppressed by serU suppressor (supH)
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- thyA6-- : Even with Luria or other complete media, supplement with thymine.
- thyA6-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- rfbC1 was formerly called rfbD1
- thiE1 was formerly called thi-1
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- strR = streptomycin resist.
- ts = Temperature Sensitive
References: - Wechsler, JA, JD Gross 1971. Escherichia coli mutants temperature-sensitive for DNA synthesis. Mol. Gen. Genet. 113(3):273-84.