CGSC Strain#: 7538
Strain Designation: DL323      Source of Strain: D.M. LeMaster
Sex: F- Chromosomal Markers: sdh-1, λ^-, mdh-2, rph-1
Strain Comments:

• For NMR relaxation technique using partially deuterated C13-glycerol and an expression plasmid
• mdh-2-- It has been noted that mdh mutants in general are leaky and are distinguished from wild-type alleles by colony size at 24-46 hours on media containing malate (autoclaved and at pH 6.0).
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)

References:

• LeMaster, D.M., D.M. Kushlan 1996. Dynamical mapping of E.coli thioredoxin via 13C NMR relaxation analysis. J.Am.Chem.Soc. 118:9255