CGSC Strain#: 5057
Strain Designation: JP5055     
Source of Strain: R.R.B. Russell
Sex: F-
Chromosomal Markers: araC14,
leuB6(Am)
,
secA206(aziR)
,
fhuA23,
lacY1,
tsx-67,
purE42,
glnX44(AS)
,
nagB2,
λ-,
trpE38,
rpsL109(strR)
,
xylA5,
mtl-1,
thiE1Strain Comments: - Probably carries malA1 lambda-resistant and a suppressor of the Mal- phenotype that maps near his.
- This strain may also carry a RifR mutation.
- leuB6(Am)-- Suppressed by serU suppressor (supH)
- purE42-- :Strains with this marker are derivatives of Χ148 and may carry malA38LAMR and a suppressor of the Mal- phenotype, sup-78,near his.
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- aziR = azide resistance
- strR = streptomycin resist.
References: - White, R.J. 1968. Control of amino sugar metabolism in Escherichia coli and isolation of mutants unable to degrade amino sugars. Biochem.J. 106:847-858
- Holmes, RP, RR Russell 1972. Mutations affecting amino sugar metabolism in Escherichia coli K-12. J. Bacteriol. 111(1):290-1.